A. Karkhaneh, H. Mirzadeh, and A.R. Ghaffariyeh (Iran)
Two-step plasma treatment, modification, collagen immobilization
The goals of this study are the surface modification of chemically inert polydimethyl siloxane (PDMS) simultaneously with acrylic acid (AAc) and 2 hydroxyethyl methacrylate (HEMA) using a method namely “two-step plasma treatment (TSPT)”; and immobilization of collagen onto this new surface. In the first step of TSPT, the films were pre-treated with oxygen plasma and immersed in the aqueous monomer solution of HEMA/ AAc mixture. Next, the second step was carried out by plasma copolymerizing of pre-adsorbed reactive monomers on the surfaces of dried pre-treated films. Then, type I collagen was linked to the carboxylic group of grafted copolymer. The films were characterized by attenuated total reflection Furrier transformer infrared (ATR-FTIR) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), zeta potential, and water contact angle measurements. The results of in vitro assays indicated a significant increase in the number of attached and proliferated cells onto modified PDMS in comparison with unmodified PDMS.
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