Two-Step Plasma Surface Modification of PDMS with Mixture of HEMA and AAc: Collagen Immobilization and In Vitro Assays

A. Karkhaneh, H. Mirzadeh, and A.R. Ghaffariyeh (Iran)

Keywords

Twostep plasma treatment, modification, collagen immobilization

Abstract

The goals of this study are the surface modification of chemically inert polydimethyl siloxane (PDMS) simultaneously with acrylic acid (AAc) and 2 hydroxyethyl methacrylate (HEMA) using a method namely “two-step plasma treatment (TSPT)”; and immobilization of collagen onto this new surface. In the first step of TSPT, the films were pre-treated with oxygen plasma and immersed in the aqueous monomer solution of HEMA/ AAc mixture. Next, the second step was carried out by plasma copolymerizing of pre-adsorbed reactive monomers on the surfaces of dried pre-treated films. Then, type I collagen was linked to the carboxylic group of grafted copolymer. The films were characterized by attenuated total reflection Furrier transformer infrared (ATR-FTIR) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), zeta potential, and water contact angle measurements. The results of in vitro assays indicated a significant increase in the number of attached and proliferated cells onto modified PDMS in comparison with unmodified PDMS.

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