E. Loose and S.J. Setford (UK)
Enzyme biosensor, stabilization, hypersolutes.
Compatible solutes from hyperthermophilic bacteria, called hypersolutes, are very efficient for the preservation of the performance of a wide variety of biomaterials; ranging from proteins to whole cells and artificial tissues. The overall objectives of this work have been to investigate the application of hypersolutes to enhance the performance of enzyme based biosensors based on the stabilization properties offered by hypersolute compounds, particularly with respect to storage and operational lifetime. Based on a simple, rapid and low cost measurement system, the optimum working conditions for the hypersolutes were determined for the following enzymes, selected due to their commercial importance: Glucose Oxidase (GOx), Alcohol Oxidase (AOx), Acetylcholinesterase (AchE) and Lactate Dehydrogenase (LDH). The sensor range, operational stability, storage stability, pH tolerance and thermal stability have been investigated. Ectoine and Firoin A presented a small stabilizing effect (+80%) against ionic strength variations. DGP gave the best stabilization against storage (+237%) with Firoin A and Hydroxyectoin (+218% and +176% respectively).
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