Bioaugmentation for Enhanced Denitrification in a Lab-Scale Treatment System

S. Andersson and G. Dalhammar (Sweden)


Bioaugmentation, denitrification, fluorescent in situ hybridization, immobilization, wastewater treatment, Comamonas denitrificans


The effect of bioaugmentation was investigated in a predenitrification lab-scale wastewater treatment system. The aim was to investigate the difference between two approaches to bioaugmentation: one in which suspended overnight culture was used as inoculum and another where bacteria immobilized in 1% agar beads were used. Pure cultures of the denitrifying bacteria Comamonas denitrificans ATCC 700936T were used in the experiments. The effect of bioaugmentation on the system was monitored using fluorescent in situ hybridization (FISH) and denitrification activity tests. The bioaugmentation with suspended bacteria showed a rapid initial (4 days) increase in denitrification activity. After 8 days the activity declined to the level of the reference system and cells of C. denitrificans were no longer detectable. Augmentation with agar-embedded bacteria resulted in a small increase in activity and very few bacteria of C. denitrificans could be observed.

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