A Novel Approach to Neurite Tracing in Fluorescence Microscopy Images

E. Meijering (The Netherlands), M. Jacob, J.-C.F. Sarria, and M. Unser (Switzerland)


Biomedical imaging, image segmentation, neurite tracing, fluorescence microscopy.


In this paper we propose a new, interactive technique for the segmentation of elongated structures in images. It is based on the so-called live-wire segmentation paradigm and uses a newly developed steerable filter for computing local ridge strength and orientation. We describe the principles of our segmentation technique and present the results of prelimi nary experiments demonstrating its potential for the tracing of neurites in fluorescence microscopy images.

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