Use of IR-to-Visible Upconversion Fluorescent Nanoparticles for Tracking of siRNA Delivery

S. Jiang and Y. Zhang (Singapore)

Keywords

upconversion nanoparticles, siRNA delivery, imaging, biomaterials

Abstract

IR-to-visible upcoversion fluorescent nanoparticles (UCNs) were developed to track in vitro delivery of small interference RNA (siRNA) and meanwhile efficiently transfect siRNA to the cells. NaYF4 upconversion nanoparticles co-doped with Yb/Tm (NaYF4:Yb,Tm) were conjugated with GL3 siRNA through electrostatic attraction at the weight ratio of 8:3. Then, siRNA conjugated UCNs were incubated with the cultured human breast cancer MCF-7 cells. After 24h-incubation the successful cellular uptake of siRNA was visualized through the emitted blue fluorescence of siRNA conjugated UCNs under confocal laser scanning microscopy. After 48h-incubation the efficiency of delivered GL3 siRNA in the inhibition of luciferase gene was ~60% that was determined by luciferase assay. This article provided initial insights into the effectiveness and suitability of NaYF4:Yb,Tm upconversion nanoparticles as an easily detectable and self-tracking delivery vector of siRNA.

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